TABLE 1.

Secretion yields of unfused scFv/scTCRs and scFv/scTCR-GFP fusions

scFv/scTCR class	Secretion level unfused (μg/liter)a	Fusion type	Secretion level fused (μg/liter)a
OX26	50 ± 7	I	69 ± 44
		II	48 ± 34
		III	60 ± 44
LWHI	2,100 ± 100	I	1,200 ± 200
		II	1,100 ± 200
		III	790 ± 260
hAb2	75 ± 6	I	41 ± 5
		II	35 ± 8
		III	51 ± 2
hAb3	4,800 ± 200	I	1,300 ± 500
		II	4,000 ± 300
		III	1,800 ± 100
hAb4	80 ± 16	I	40 ± 8
		II	20 ± 11
		III	55 ± 14
hAb7	81 ± 12	I	270 ± 100
		II	140 ± 78
		III	160 ± 44
hAb8	23 ± 11	I	15 ± 10
		II	4 ± 3
		III	27 ± 14
hAb10	20 ± 8	I	36 ± 8
		II	13 ± 10
		III	20 ± 7
scFv A	4,000 ± 1,100	I	1,500 ± 150
		II	2,900 ± 900
		III	2,400 ± 700
yEGFP	3,000 ± 145	NAb	NA

^aTriplicate cultures were analyzed for each construct, and values represent means±standard deviations. The final cell density was nearly the same for each construct, and therefore, comparison by volumetric yields is similar to comparison by per-cell yields.

^bNA, not applicable.