TABLE 2.
Intracellular accumulation of full-length fusion proteins and unfused scFv/scTCR and GFP
| ScFv/scTCR class | Unfused
|
Fused
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Secretion level (μg/liter) | Intracellular (μg/liter)a | Intracellular/ secretion | Fusion type | Secretion level (μg/liter) | Intracellular (μg/liter)a | Intracellular/ secretion | Relative fluorescence/moleculeb
|
||
| Intracellular | Secreted | ||||||||
| hAb3 | 4,800 ± 200 | 7,700 ± 1,600 | 1.6 ± 0.3 | I | 1,300 ± 500 | 15,000 ± 1,000 | 11 ± 0.8 | 1.00 ± 0.02 | 1.00 ± 0.01 |
| II | 4,000 ± 300 | 11,000 ± 2,900 | 2.6 ± 0.7 | 1.35 ± 0.09 | 1.57 ± 0.23 | ||||
| III | 1,800 ± 100 | 15,000 ± 800 | 8.4 ± 0.5 | 2.20 ± 0.04 | 2.19 ± 0.19 | ||||
| hAb7 | 81 ± 12 | 5,400 ± 800 | 67 ± 10 | I | 270 ± 100 | 15,000 ± 2,000 | 55 ± 7 | 1.00 ± 0.02 | ND |
| II | 140 ± 78 | 9,200 ± 200 | 66 ± 14 | 1.89 ± 0.21 | ND | ||||
| III | 160 ± 44 | 12,000 ± 700 | 75 ± 4 | 6.25 ± 0.78 | ND | ||||
| hAb8 | 23 ± 11 | 6,700 ± 1,900 | 290 ± 83 | I | 15 ± 10 | 14,000 ± 4,500 | 940 ± 300 | 1.00 ± 0.08 | ND |
| II | 4 ± 3 | 7,300 ± 2,000 | 1,800 ± 500 | 2.70 ± 0.22 | ND | ||||
| III | 27 ± 14 | 12,000 ± 1,600 | 450 ± 59 | 4.19 ± 0.37 | ND | ||||
| LWHI | 2,100 ± 100 | 1,500 ± 500 | 0.7 ± 0.2 | I | 1,200 ± 200 | 15,000 ± 5,000 | 12.5 ± 4.2 | 1.00 ± 0.02 | 1.00 ± 0.06 |
| II | 1,100 ± 200 | 11,600 ± 2,500 | 10.5 ± 2.3 | 2.11 ± 0.30 | 2.04 ± 0.27 | ||||
| III | 790 ± 260 | 9,900 ± 1,500 | 23.1 ± 1.9 | 6.95 ± 0.47 | 3.61 ± 0.37 | ||||
| YEGFP | 3,000 ± 145 | 11,300 ± 1,900 | 3.8 ± 0.6 | ||||||
a
Triplicate cultures were analyzed for each condition, and all secretion and intracellular levels were determined by Western blotting. Values represent means±standard deviations.
b
Relative fluorescence yields were normalized to the type I fusion protein for each species. ND, not determined.