Table 1.
Pchlide-dependent import of pPORA into chloroplasts of monocotyledonous and dicotyledonous plant species
| Plant species | Import, % of added precursor
|
|||
|---|---|---|---|---|
| pPORA
|
pPORB
|
|||
| −Pchlide | +Pchlide | −Pchlide | +Pchlide | |
| Monocotyledonous | ||||
| Barley | n.d. | 62 | 58 | 56 |
| Oat | n.d. | 48 | 52 | 54 |
| Wheat | n.d. | 65 | 56 | 54 |
| Dicotyledonous | ||||
| Pea | n.d. | 40 | 34 | 38 |
| Bean | n.d. | 42 | 36 | 32 |
| Arabidopsis | n.d. | 38 | 32 | 32 |
| Tobacco | n.d. | 32 | 30 | 28 |
| Spinach | n.d. | 34 | 28 | 26 |
Import reactions were carried out at 23°C in the dark with 2 mM Mg-ATP and chloroplasts containing (+Pchlide) or lacking (−Pchlide) Pchlide produced by 5-ALA pretreatment. Percentages refer to the levels of imported, thermolysin-resistant mature PORA and PORB, relative to the levels of added precursors per assay, after a 15-min import reaction. n.d., not detectable.