Table 2.

Blocking TOC86 or TOC75 does not inhibit import of pPORA into Pchlide-containing barley chloroplasts

Precursor	Import, % of added precursor
	transA-DHFR		transB-DHFR		transPC-DHFR
	−Pchlide	+Pchlide	−Pchlide	+Pchlide	−Pchlide	+Pchlide
anti-TOC86	n.d.	64	2	4	5	3
anti-TOC75	n.d.	62	8	6	4	6

Protochlorophyllide-containing (+Pchlide) and Pchlide-free (−Pchlide) chloroplasts bearing anti-TOC86 or anti-TOC75 Fab fragments were incubated with the indicated radiolabeled precursors at 23°C in the dark in the presence of 2 mM Mg-ATP. Quantification of the import data was performed as described in Table 1, but percentages refer to mature DHFR levels relative to added precursor levels, respectively, per assay. n.d., not detectable.