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Detection of intracytoplasmic IFN-γ in whole-blood cultures. Blood taken from M. bovis-infected animals 12 weeks postinfection was cultured in vitro for 24 h with PBS (a), PPD-B (b), BCG (c), or M. bovis (d). Expression of intracellular IFN-γ within CD4+ T cells was examined by flow cytometry. Percentages of cells in each quadrant are illustrated. One representative animal of three is shown.
