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. 2000 Aug 8;97(17):9807–9812. doi: 10.1073/pnas.170286897

Figure 5.

Figure 5

NLS mutations and yeast nuclear localization assay. (A) Amino acid replacements at NLS 1, 2, and 3 are underlined. Only regions of NLS motifs are shown. KKKRK was introduced into AvrXa7 M123 to create AvrXa7SV40. (B) Each gene was fused to the LexA coding DNA-binding domain in pNIA vector and expressed in yeast. Strain L40 contains LexA-binding sites (UASLexA) immediately upstream of lacZ and His 3 genes. Induction of lacZ and His3 depends on the nuclear localization of the LexA fusion product. Induction leads to increased β-galactosidase activity and the ability of the yeast strain to grow in histidine-deficient media.