Figure 7.

AvrXa7 is a DNA-binding protein. (A) Single-stranded and double-stranded oligonucleotides were end labeled with ³²P, mixed with AvrXa7 or GST (glutathione S-transferase), and subjected to polyacrylamide electrophoresis. Arrow indicates position of well. (B) DNA-binding competition to AvrXa7. Random oligonucleotides were labeled with ³²P and mixed with AvrXa7 and the indicated unlabeled DNA in 100-fold excess. A, T, C, G, indicate unlabeled single-stranded homopolymers; A/T and C/G indicate unlabeled double-stranded polymers. +/− indicate presence or absence of component.