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Effect of nls123 mutations on nuclear localization in yeast assay
| Gene | Histidine deficiency* | % β-galactosidase activity† |
|---|---|---|
| pNIA | − | 3.1 ± 1.1 |
| pNIAXa7 | + | 100 |
| pNIAXa7M123 | ± | 20.0 ± 3.8 |
| pNIAXa7SV40 | + | 76.3 ± 2.4 |
Histidine deficiency was measured as the ability to grow on minimal media with 20 mM 3-AT
Activity is expressed as percent of activity of o-nitrophenyl β-d-galactopyranoside (ONPG) produced per hour by pNIAXa7.
