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The replacement vectors for the pel genes in N. hematococca. Open boxes, the 5′ and 3′ flanking regions of the pel genes; black boxes, the pel gene exons; restriction sites, H, HindIII; X, XbaI; and S, SstI. Primers 1 and 2 were used for detecting homologous recombination of hph-disrupted pelD and primers 2 and 3 for zeo-disrupted pelD.
