Figure 4.

Infection of pea epicotyl sections by (a) wild type; (b) pelA disruptant; (c) pelD disruptant; (d) pelA, pelD disruptant; (e) pelD-complemented pelA, pelD disruptant of N. hematococca; (f) infection droplets of pelA, pelD double-disruptant supplemented with PLA; or (g) double disruptant supplemented with PLD – lesion formation is seen in all cases except with pelA, pelD double-gene disruptant. (h) Mycelial penetration of pea epicotyl sections inoculated with the wild type or the indicated single and double disruptants. (i) Penetration by the wild-type, double-disruptant, three pelD-complemented double disruptants (C-1, C-2, and C-3), and double-disruptant spore suspension supplemented with purified PLA (100 ng) or PLD (40 ng). Assay with 10⁶ conidia (28).