FIG. 2.

Characterization of the pex5Δ/pex5Δ and fox2Δ/fox2Δ strains by immunoelectron microscopy. Oleate-induced wild-type cells (A and E), pex5Δ/pex5Δ cells (B and F), fox2Δ/fox2Δ cells (C and G), or complemented pex5Δ/pex5Δ cells (D and H) were fixed and prepared for immunocytochemistry. Cryosections were incubated with antibodies directed against catalase (A to D) or thiolase (E to H), and antigens were visualized with immunogold particles conjugated to protein A. P, peroxisome, M, mitochondrion, N, nucleus. Bars, 0.5 μm.