FIG. 1.

Effect of GR deficiency on the growth, viability, and cell cycle arrest in S. pombe. (A) A seed culture of JL36 (nmt1-pgr1) cells grown overnight was inoculated to an OD₅₉₅ of 0.01 in EMM in the presence (square) or absence (diamond) of 10 μM thiamine. The growth was monitored at 30°C by measuring the OD₅₉₅. Growth analyses were carried out at least three times independently for each strain, and a representative growth curve for each condition is shown. (B) At around the time of growth arrest (13, 16, and 19 h postinoculation), cells were diluted and plated onto EMM plates in triplicate to monitor viable cell counts. (C) At the arrested time point (16 h postinoculation), cells were harvested and fixed with ethanol. After removing RNA, the remaining nucleic acid was stained with PI and subjected to fluorescence-activated cell sorter analysis. x and y axes represent fluorescence (DNA content) and cell number, respectively. The upper panel represents patterns for control cells of 1C or 2C DNA content. The lower panel represents results for JL36 cells grown in the absence (−) and presence (+) of thiamine (thi).