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. 2006 Sep 1;5(11):1914–1924. doi: 10.1128/EC.00263-06

TABLE 1.

The yeast strains used in this study

Strain Genotype Reference
Strains with a single gene for wild-type or T22I mutant yeast Hsp90
    P82a W303-1a hsc82::LEU2 hsp82::LEU2 HIS3-HSP82a 38
    T22I W303-1a hsc82::LEU2 hsp82::LEU2 HIS3-hsp82(T22I)a 38
    P82aslt2Δ P82a slt2ΔkanMX4 34
    T22Islt2Δ T22I slt2ΔkanMX4 34
    PP30slt2Δ MATatrp1-289 leu2-3,112 his3-200 ura3-52 ade2-101oc lys2-801am hsc82::kanMX4 hsp82::kanMX4 slt2Δ::hphMX4 [pHSC82]b This study
    PP30 mkk1Δ mkk2Δ slt2Δ MATatrp1-289 leu2-3,112 his3-200 ura3-52 ade2-101oc lys2-801am hsc82::kanMX4 hsp82::kanMX4 mkk1::kanMX4 mkk2::HIS3 slt2Δ::hphMX4 [pHSC82]b This study
Two-hybrid strains
    PJ694a MATatrp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ 21
    PJ694α MATα trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ 21
a

The only Hsp90 gene in these strains, an integrated wild-type (HSP82) or mutant (hsp82) gene expressed from the constitutive TPD1 gene promoter (38).

b

The only Hsp90 gene in these strains, a wild-type HSC82 under native promoter control on a URA3 vector (44).