TABLE 2.
Alignment of the activation loop regions of the five yeast MAPKs, human ERK5, and human ERK2
| MAPK | Sequencea |
|---|---|
| Kss1p | DFGLARCLASSSDSRETLVG---FMTEYVATRWYRAPE |
| Fus3p | DFGLARIIDESAADNSEPTGQQSGMTEYVATRWYRAPE |
| Smk1p | DFGLARGIHAGFFKCHSTVQP--HITNYVATRWYRAPE |
| Hog1p | DFGLARIQDPQ-------------MTGYVSTRYYRAPE |
| Slt2p | DFGLARGYSENPVENSQ------FLTEYVATRWYRAPE |
| ERK5 | DFGMARGLCTSPAEHQY------FMTEYVATRWYRAPE |
| ERK2 | DFGLARVADPDHDHTG-------FLTEYVATRWYRAPE |
| Identityb | ***:** :* **:**:***** |
a
A G. …P…E motif common to Slt2p and ERK5 is in boldface type; the TXY motif that is dually Thr/Tyr phosphorylated by the activating MAP2K is underlined.
b
A highly flexible loop anchored between conserved DFG and APE tripeptide sequences in protein kinases (39).