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HA-Snf1-T210A does not support PH differentiation. Strain KY40 (snf1Δ/snf1Δ) was transformed with pMO18 expressing HA-Snf1, pMO19 expressing HA-Snf1-T210A, or with the parent vector pSK134HA (Vector). The wild-type control strain MCY4472 (WT) carried the empty vector pSK134HA. All transformants also carried pRS316 (62), a centromeric vector with URA3, to provide prototrophy. The strains were examined on SLAD plates for PH differentiation. Colonies were photographed after 4 days at 30°C.
