FIG. 3.

MPP2-mediated proteolytic processing is responsible for the enhanced cleavage phenotype exhibited by ΔMIC5-1 parasites. Western blots of ESA fractions from RH or ΔMIC5-1 parasites treated with the protease inhibitor ALLN or dimethyl sulfoxide (DMSO, solvent control) and probed with anti-SUB1, anti-GRA1, or anti-AMA1. ALLN reverses the enhanced processing of SUB1 and GRA1. Gray and black arrows denote GRA1 fragments generated by ALLN-insensitive and -sensitive events, respectively. AMA1 is included as a loading control.