FIG. 1.

Overexpression of Rav1p is toxic. (A and B) CRY1 (wild type) cells were transformed either with p413GAL or p413GAL.RAV1, and GSY11-1A (rav1Δ) was transformed with p413GAL. The p413GAL plasmid possesses the galactose-inducible GAL1 promoter. Strains were tested on SC-His (glucose) and SGC-His (galactose) for the ability to grow when Rav1p was overexpressed. (C) CRY1 (wild type) cells transformed with p413GAL.RAV1 were grown in log phase in either SSC-His (Sucrose) or SGC-His (galactose). Strains were visualized under differential interference contrast microscopy after 24 h.