FIG. 6.

Sucrose density gradient analysis of hydrogenosomal lysates. One milligram of hydrogenosomal extract (1.0 ml) was loaded onto linear sucrose gradients (2 to 20%) in 20 mM Tris-HCl at pH 7.5 containing 1% Triton X-100, 250 mM NaCl, TLCK, and leupeptin. One-milliliter fractions were collected, TCA precipitated, subjected to 15% SDS-PAGE, and analyzed by immunoblotting using anti-L and anti-H antibodies. Band intensity was plotted against fraction number. Arrows indicate the positions of molecular mass standards (soybean trypsin inhibitor, 20.1 kDa; bovine serum albumin, 67 kDa; lactate dehydrogenase, 140 kDa; catalase, 232 kDa; ferritin, 440 kDa).