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Kinetics parameters for L protein, using different concentrations of H protein and lipoamide as substratesa
| Compound | Apparent Km (μM) | Apparent Vmax (nmol/s) | kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
| rTvH1 protein | 2.6 | 84.07 | 0.84 | 3.2 × 105 |
| rTvH2 protein | 3.7 | 208.03 | 2.08 | 5.6 × 105 |
| Lipoamide | 34.0 | 60.26 | 0.602 | 1.7 × 104 |
The enzymatic assay was performed as described in Materials and Methods. Kinetic parameters were calculated by using varying concentrations of the two H proteins and lipoamide.
