TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strainsa | ||
| 10560-2B | MATaura3-52 his3::hisG leu2::hisG | G. R. Fink |
| 10560-5B | MATα ura3-52 trp1::hisG leu2::hisG | G. R. Fink |
| JK353 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG | This laboratory |
| JK354 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG kem1::LEU2/kem1::LEU2 | This laboratory |
| JK371 | MATaura3-52 his3::hisG leu2::hisG FLO11::HA | This work |
| JK372 | MATaura3-52 his3::hisG leu2::hisG GPA2::HA | This work |
| JK373 | MATaura3-52 his3::hisG leu2::hisG STE12::HA | This work |
| JK374 | MATaura3-52 his3::hisG leu2::hisG CLN1::HA | This work |
| JK375 | MATaura3-52 his3::hisG leu2::hisG FLO8::HA | This work |
| JK376 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/leu2::hisG/leu2::hisG FLO11/FLO11::HA | This work |
| JK377 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG GPA2/GPA2::HA | This work |
| JK378 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG STE12/STE12::HA | This work |
| JK379 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG CLN1/CLN1::HA | This work |
| JK380 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG FLO8/FLO8::HA | This work |
| JK381 | MATaura3-52 his3::hisG leu2::hisG caf20::LEU2 | This work |
| JK383 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG caf20::LEU2/caf20::LEU2 | This work |
| JK384 | MATaura3-52 his3::hisG leu2::hisG tif1::LEU2 | This work |
| JK386 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG tif1::LEU2/tif1::LEU2 | This work |
| JK387 | MATaura3-52 his3::hisG leu2::hisG dhh1::LEU2 | This work |
| JK389 | MATa/MATα ura3-52/ura3-52 his3::hisG/+ trp1::hisG/+ leu2::hisG/leu2::hisG dhh1::LEU2/dhh1::LEU2 | This work |
| Plasmids | ||
| pJI255 | STE12-HA URA3 CEN | This work |
| pJI256 | CLN1-HA URA3 CEN | This work |
| pJI257 | FLO8-HA URA3 CEN | This work |
| pJI274 | STE12-HA 2μ ARS URA3 | This work |
| pJI276 | CAF20 URA3 CEN | This work |
| pJI277 | DHH1 URA3 CEN | This work |
| YEp355-FLO11::lacZ | FLO11::lacZ URA3 2μ | 37 |
| pRS426 | 2μ ARS URA3 | 6 |
| pRS316 | CEN ARS URA3 | 42 |
a
All yeast strains are derived from the Σ1278b background.