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Kinetic characterization of rTbHKs
| Protein | Apparent Km (mM)a
|
||||
|---|---|---|---|---|---|
| Glucose | ATP | Mannose | Fructose | MgCl2 | |
| rTbHK1 | 0.09 ± 0.02 | 0.28 ± 0.1 | 0.03 ± 0.01 | 0.35 ± 0.3 | 0.92 ± 0.21 |
| rTbHK2 | ND | ND | ND | ND | ND |
| rTbHK1C2 | ND | ND | ND | ND | ND |
| rTbHK2C1 | 0.07 ± 0.006 | 0.44 ± 0.11 | 0.05 ± 0.01 | NA | 0.58 ± 0.2 |
| rTbHK1(N469D) | ND | ND | ND | ND | ND |
| Yeast Hxk | 0.18 ± 0.1 | 0.25 ± 0.04 | NA | NA | 2.2 ± 0.42 |
Data are means ± standard deviations. ND, not determined, as activity was not detected; NA, not assayed.
