TABLE 4.
Xylanase and cellulase activities in supernatants from direct cultivations of the parental strain and the Δxyr1 strains with xylan or cellulose as respective carbon sources
Cultivation time (h) | Xylanase activity (U/μg protein) in xylan supernatantsa,c
|
Cellulase activity (U/μg protein) in cellulose supernatantsb,c
|
||
---|---|---|---|---|
QM9414 | Δxyr1 | QM9414 | Δxyr1 | |
24 | NDd | ND | 0.003 ± 0.000 | ND |
48 | 0.206 ± 0.015 | ND | 0.655 ± 0.012 | ND |
72 | 0.142 ± 0.009 | ND | 0.885 ± 0.022 | ND |
One unit of activity is defined as the amount of enzyme required to release 1 micromole of xylose reducing sugar equivalents per min under the defined assay conditions.
One unit of activity is defined as the amount of enzyme required to release 1 micromole of glucose reducing sugar equivalents per min under the defined assay conditions.
Values are the means ± SD of results from three independent experiments.
ND, no detection.