TABLE 3.
Changes in expression of metabolic genes in an ace2 deletion strain under yeast- and hypha-inducing conditions
| Systematic name and metabolic categorya | Standard nameb | Description | Change in expression (fold)c
|
|
|---|---|---|---|---|
| Yeastd | Hyphae | |||
| Glycolysis and reserve carbohydrates | ||||
| orf19.5348 | TPS3 | Regulatory subunit of trehalose-phosphate synthase | −3.0 | −1.9 |
| orf19.7021 | GPH1 | Glycogen phosphorylase | −2.4 | — |
| orf19.3278 | GSY1 | Glycogen synthase | −4.8‡ | −1.8 |
| orf19.3325 | GLG2 | Self-glucosylating initiator of glycogen synthesis | −3.2 | −1.5 |
| orf19.2020 | HXT6 | Glucose transporter | −2.4 | −2.0 |
| orf19.542 | HXK2 | Hexokinase II | −2.3 | −1.5 |
| orf19.3888 | PGI1 | Glucose-6-phosphate isomerase | −1.8 | −1.6 |
| orf19.3967 | PFK1 | Phosphofructokinase (alpha subunit) | — | −1.7 |
| orf19.6540 | PFK2 | Phosphofructokinase (beta subunit) | −2.1 | — |
| orf19.4753 | PFK26 | 6-Phosphofructose-2-kinase | −1.5 | — |
| orf19.6814 | TDH3 | Glyceraldehyde-3-phosphate dehydrogenase | −1.7‡ | −1.7 |
| orf19.3651 | PGK1 | 3-Phosphoglycerate kinase | −1.9‡ | −1.7 |
| orf19.4618 | FBA1 | Fructose-bisphosphate aldolase | −2.0 | −1.5 |
| orf19.903 | GPM1 | Phosphoglycerate mutase | −1.6 | −1.6 |
| orf19.395 | ENO1 | Enolase I | −1.8‡ | −1.6 |
| orf19.5113 | ADH2 | Alcohol dehydrogenase | −2.0 | −1.9 |
| orf19.3997 | ADH1 | Alcohol dehydrogenase | −1.8 | — |
| Gluconeogenesis | ||||
| orf19.6178 | FBP1 | Fructose-1,6-bisphosphatase | — | +1.8 |
| orf19.7514 | PCK1 | Phosphoenolpyruvate carboxykinase | — | +3.7 |
| TCA cycle | ||||
| orf19.3097 | PDA1 | Pyruvate dehydrogenase (alpha subunit) | — | +1.9 |
| orf19.4393 | CIT1 | Citrate synthase | +2.5‡ | +2.3 |
| orf19.6385 | ACO1 | Aconitase | +1.5‡ | +4.3 |
| orf19.4826 | IDH1 | Isocitrate dehydrogenase subunit | — | +1.9 |
| orf19.5791 | IDH2 | Isocitrate dehydrogenase subunit | — | +2.75 |
| orf19.6165 | KGD1 | Alpha-ketoglutarate dehydrogenase | +1.5‡ | +1.8 |
| orf19.3358 | LSC1 | Succinyl-CoA ligase (alpha subunit) | — | +1.5 |
| orf19.4022 | SDH4 | Succinate dehydrogenase | +1.6‡ | — |
| orf19.543 | FUM1† | Fumarate hydratase | +1.6 | +1.6 |
| Oxidative phosphorylation and ATP synthesis | ||||
| orf19.3577 | COQ5 | Methyltransferase of ubiquinone (coenzyme Q) biosynthesis | +1.5 | — |
| orf19.3008 | COQ4 | Ubiquinone biosynthesis | +1.6 | — |
| COQ6 | Ubiquinone biosynthesis | — | −1.5 | |
| orf19.7049 | CYB5 | Cytochrome b5 | +1.7 | — |
| orf19.2201 | CBP6† | Cytochrome b protein synthesis | +1.9 | — |
| orf19.1770 | CYC1 | Cytochrome c | +2.0 | — |
| orf19.3527 | CYT1 | Cytochrome c1 | — | +1.6 |
| orf19.4578 | CYT2 | Cytochrome c1 heme lyase | +1.8 | — |
| orf19.1416 | COX11 | Cytochrome c oxidase assembly | +1.7 | — |
| orf19.2006.1 | COX17† | Cytochrome c oxidase assembly | +2.3 | — |
| orf19.3946 | COX18† | Cytochrome c oxidase assembly | +1.8 | — |
| orf19.4967 | COX19 | Required for cytochrome c oxidase activity | +1.6‡ | — |
| orf19.2644 | QCR2 | Ubiquinol-cytochrome c reductase 40-kDa chain II | — | +2.1 |
| orf19.5893 | RIP1 | Ubiquinol cytochrome c reductase | — | +1.9 |
| orf19.4929 | PET309† | Translational activator of COX1 | +1.6 | — |
| orf19.7577 | MSS51 | Required for translation of COX1 | +2.3 | — |
| orf19.6565 | OXA1† | Assembly of cytochrome c oxidase, ATP synthase, and ubiquinol-cytochrome c oxidoreductase | +2.2 | +1.4 |
| orf19.6854 | ATP1 | F1Fo ATPase complex | — | +1.9 |
| orf19.5653 | ATP2 | F1Fo ATPase complex | — | +2.3 |
| orf19.3579 | ATP4 | F1FoATPase complex | — | +1.7 |
| orf19.5419 | ATP5 | F1Fo ATPase complex | — | +1.8 |
| orf19.2785 | ATP7 | F1Fo ATPase complex | +1.7‡ | +2.0 |
| CA4830* | ATP8 | F1Fo ATPase complex | +1.4 | — |
| orf19.6916 | ATP11† | F1Fo ATPase complex | +2.0 | — |
| orf19.3686 | ATP12 | F1Fo ATPase complex | +1.6‡ | — |
| Lipid and fatty acid metabolism | ||||
| orf19.5577 | YDR531W† | Pantothenate kinase | +1.7 | −1.7 |
| orf19.3171 | ACH1 | Acetyl-CoA hydrolase | +2.0 | — |
| orf19.7466 | ACC1 | Acetyl-CoA carboxylase | +1.9 | — |
| orf19.7043.1 | ACB1 | Acyl-CoA binding protein | +1.4 | −1.6 |
| ARE2 | Acyl-CoA:sterol acyltransferase | — | −2.8 | |
| orf19.979 | FAS1 | Fatty acid synthase (beta subunit) | +1.7 | — |
| orf19.5949 | FAS2 | Fatty acid synthase (alpha subunit) | +1.5 | −1.6 |
| orf19.7592 | FAA4 | Long-chain-fatty acyl-CoA synthetase | +1.4 | — |
| orf19.5818 | SUR2 | Hydroxylation of C-4 of sphingoid moiety of ceramide | — | −1.9 |
| orf19.6860 | PIS1 | Phosphatidylinositol synthase | +1.5 | — |
| Ergosterol metabolism | ||||
| orf19.406 | ERG1 | Squalene epoxidase | — | −1.9 |
| orf19.6026 | ERG2 | C-8 sterol isomerase | +1.6 | — |
| orf19.5178 | ERG5 | C-22 sterol desaturase | — | −1.5 |
| orf19.3616 | ERG9 | Farnesyl-diphosphate farnesyltransferase | +1.3‡ | — |
| orf19.1598 | ERG24 | C-14 sterol reductase | +1.3 | — |
| orf19.1591 | ERG10 | Acetyl-CoA acetyltransferase | +1.7 | — |
| orf19.922 | ERG11 | Lanosterol 14-alpha-demethylase | — | −2.4 |
| orf19.4631 | ERG251 | C-4 methylsterol oxidase activity | — | −1.7 |
a
The systematic (orf19) name from http://www.candidagenome.org is used where possible; one ORF was not represented in the Candida Genome Database, and the CandidaDB (http://genolist.pasteur.fr/CandidaDB/) designation was used (asterisk).
b
The standard name from the Candida Genome Database is used where possible; where the standard name is the same as the systematic name, the name of the S. cerevisiae ortholog was substituted (†).
c
—, changes in gene expression were not statistically reproducible with the methods used. This does not suggest that gene expression is unchanged.
d
The genes with altered expression in yeast cells were mostly obtained using an FDR of 0.23%. The indicated genes (‡) were present only at an FDR of 0.99%.