TABLE 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| yMC286 | MATahis3 leu2 ura3 Rpb7-GFP-HIS3X6 | This work |
| yMC310 | MATahis3 leu2 ura3 rpb4Δ::HIS3 Rpb7-GFP-HIS3X6 | This work |
| PSy413 | MATα his3 leu2 ura3 ade2 ade3 nup49::TRP1 + pUN100 (CEN/LEU2/nup49-313) | 15 |
| yMC190 | MATarpb1-1 his3 leu2 ura3 ade3 nup49::TRP1 + pUN100 (CEN/LEU2/nup49-313) | P. Silver |
| yMS3 | PSy413 + pMC190 (CEN/URA3/GFP-RPB4) + pMC308 (CEN HIS3 RPB7-RFP) | This work |
| yMS4 | yMC190 + pMC190 (CEN URA3 GFP-RPB4) + pMC308 (CEN/HIS3/RPB7-RFP) | This work |
| yMS8 | YMC310 + pMC305 (CEN/URA3/RPB4-RFP) | This work |
| yMS18 | MATalys801 leu2-3,112 trp1-1 ura3-52 his3Δ200 + pMC190 (CEN URA3 GFP-RPB4) + MC308 (CEN/HIS3/RPB7-RFP) | This work |
| Fy86 | MATα his3Δ200 ura3-52 leu2Δ1 | 42 |
| yMS19 | Fy86 + pMC143 (CEN/LEU2/GFP-RPB4) + pMC307 (CEN URA3 RPB7-RFP) | This work |
| yMS21 | MATα ade2 ade3 his3 leu2 ura3 nsp49::TRP1 rpb4Δ1::HIS3 + pUN100 (CEN LEU2 nup49-313) + p307 (CEN URA3 RPB7-RFP) | This work |
| yMC140 | MATa rpb7Δ::LEU2 ura3-52 his3Δ200 leu2-3,112 lys2Δ201 ade2 pURA3/CEN/RPB7 | 20 |
| yRL81 | MATarpb7Δ::LEU2 ura3-52 his3Δ200 leu2-3,112 lys2Δ201 ade2 pHIS3/CEN/Rpb7-29I45T,P63T,T64S,F109S,N122S-RFP p143 (GFP-RPB4/CEN/URA3) | This work |
| yRL82 | MATarpb7Δ::LEU2 ura3-52 his3Δ200 leu2-3,112 lys2Δ201 ade2 pHIS3/CEN/RPB7-RFP p143 (GFP-RPB4/CEN/URA3) | This work |