TABLE 1.
Equilibrium association constants for complexes of Leishmania elF4E isoforms and mouse eIF4E (amino acids 28 to 217) with cap analoguesd
Cap analogue |
Kas (μM−1) for:
|
||||
---|---|---|---|---|---|
Mouse eIF4E (28-217) | LeishIF4E-1 | LeishIF4E-2 | LeishIF4E-3 | LeishIF4E-4 | |
m7GTP | 121.4 ± 3.0a | 0.16 ± 0.02 | 0.037 ± 0.006 | 0.32 ± 0.07 | 0.8 ± 0.08 |
m7GpppA | 4.4 ± 0.1a | 0.072 ± 0.002b | 0.016 ± 0.010 | 0.020 ± 0.005 | 0.21 ± 0.03 |
Cap 1 | |||||
m7Gpppm36,6,2′ A | 1.45 ± 0.03a | 0.065 ± 0.004b | 0.020 ± 0.004 | 0.029 ± 0.007 | 0.075 ± 0.031 |
Cap 2 | |||||
m7Gpppm36,6,2′ Apm2′ A | 10.7 ± 1.4 | 0.067 ± 0.005 | 0.092 ± 0.008 | 0.063 ± 0.010 | 0.23 ± 0.06 |
Cap 3 | |||||
m7Gpppm36,6,2′ Apm2′ Apm2′ C | 22.2 ± 1.0 | 0.19 ± 0.01 | 0.24 ± 0.02 | ND | ND |
Cap 4 | |||||
m7Gpppm36,6,2′ Apm2′ Apm2′ Cpm23,2′ U | 26.1 ± 0.7a | 0.253 ± 0.003b | 0.28 ± 0.04 | 0.046 ± 0.02 | 0.77 ± 0.08 |
Cap 0 | |||||
m7GpppApApCpU | 73.3 ± 1.8a | 0.467 ± 0.005c | 0.089 ± 0.013 | 0.12 ± 0.03 | 2.0 ± 0.4 |
TMG | |||||
m32,2,7GTP | 0.64 ± 0.06 | <0.01 | <0.003 | <0.04 | <0.006 |
GTP | 0.032 ± 0.004a | <0.01 | <0.003 | <0.01 | <0.01 |