FIG. 4.

Amino acid composition analyses of the predicted TPs of class I and II targeting sequences compared to the mature proteins (MP). The amino acid compositions of the intervening region between TMH1 and TMH2 of class I targeting sequences (TP, I; n = 70), the predicted transit peptide region for class II proteins (TP, II; n = 13), and the mature protein regions from class I proteins (MP, I; n = 70) were determined. Also shown are the amino acid compositions of Chlamydomonas reinhardti TPs (TP, Cr; n = 25) and mature proteins (MP, Cr; n = 25). Box-and-whisker plots were used to represent the data and are based on quartiles around the median value. The box encloses 50% of the data, with 25% above and below the median (solid line). Each whisker represents the data range of an additional 25% of the data. The existence of outliers beyond the 5% and 95% confidence ranges is indicated with a solid dot where applicable. Categories indicated with different letters on the plot are significantly different (one-way ANOVA and Tukey's test [α ≤ 0.05]). All data were normal except for the Lys content in class II peptides, in which case nonparametric statistics were used to assess differences.