TABLE 1.
Fatty acid profiles of total internal lipids extracted from U. maydis wild-type (a2b2) and mfe2 mutant (Δmfe2 a2b2) strains grown either on glucose or oleic acida
Fatty acidd | Result (%b ± SD) for strain and carbon source
|
|||
---|---|---|---|---|
a2b2
|
Δmfe2 a2b2
|
|||
Glucose | Oleic acid | Glucose | Oleic acid | |
C14:0 | 1 ± 0.2 | NDc | tre | ND |
C16:0 | 23 ± 0.5 | tr | 17 ± 2.1 | tr |
C16:1 | 1 ± 0.0 | 1 ± 0.5 | 1 ± 0.5 | 1 ± 0.0 |
C18:0 | 5 ± 1.3 | tr | 4 ± 0.5 | tr |
C18:1 | 29 ± 2.4 | 79 ± 3.3 | 20 ± 3.8 | 90 ± 0.2 |
C18:2 | 42 ± 3.0 | 20 ± 5.0 | 57 ± 4.3 | 9 ± 1.7 |
C20:0 | tr | tr | tr | ND |
The table shows data from three independent experiments.
Percentage of total fatty acids.
ND, not detected.
Internal lipids were extracted from cells grown in minimal medium supplemented with the appropriate carbon source for 20 h. Fatty acids were analyzed by gas chromatography.
tr, found in trace amounts.