TABLE 1.
Infection and inflammation in the brain of scid mice 1 month after inoculation with B. turicatae
| Inoculum | Mean spirochetes per μg of brain RNAa (95% CI) | Sum area stained for F4/80b (95% CI) |
|---|---|---|
| Serotype 1 | 40 (26-67) | 33,000 (23,000-43,000) |
| Serotype 2 | 4 (2-6) | 4,900 (3,300-6,500) |
| PBS | 0 | 1,200 (300-1,200) |
a
Results were measured by TaqMan RT-PCR amplification of 16S rRNA. P < 0.001 for the difference between serotypes 1 and 2.
b
Results are mean (95% confidence interval) sum areas in square microns per 40× microscopic field. P < 0.001 for the differences between serotype 1 and PBS as well as serotype 1 and serotype 2; P < 0.05 for the difference between serotype 2 and PBS. Activated macrophages/microglia were detected by immunostaining with rat anti-mouse F4/80 monoclonal antibody.