FIG. 2.

Pathology and recruitment of leukocytes to the liver. (A to O) H&E staining and IHC analysis of control liver tissue isolated from mice injected with Hanks balanced salt solution (O) or infected by the designated strains of Y. pseudotuberculosis (A to N). Representative images (magnification, ×400) were captured with a light microscope controlled by Image Pro plus software (EPIX, Inc., Buffalo Grove, IL). IHC staining of neutrophils (LY-6G-positive cells, detected by monoclonal antibody [MAb] NIMP-R14; Hycult Biotechnology, Uden, The Netherlands), macrophages (F4/80-positive cells, detected by rat anti-mouse F4/80 MAbs; Serotec, Raleigh, NC), and T cells (CD3-positive cells, detected by MAb MCA 1477; Serotec) is shown. All experiments were repeated three times; the data shown are from one representative experiment.