TABLE 1.
Oligonucleotides used for the construction of recombinant toxin fragments
| Toxin | Fragment | Oligonucleotidea
|
bp amplified | Amino acids | |
|---|---|---|---|---|---|
| Forward | Reverse | ||||
| A | 1 | GGTTGCTGGATCCATGTCTTTAATATCTAAAGAAGAGTTAATAAAACTCGCATATAGC | CCATGCTGAGCTCGCTTCATCTTTACCATGTCCAATAAAGGTTACTTTTACTTTTTCC | 1-1977 | 1-659 |
| A | 2 | GGTTGCTGGATCCTTCAACACAAGCGAATTTGCTAGATTAAGTGTAGATTCAC | CCATGCTGAGCTCGCTGAATCAAGTAATCTAGTTCCGTCATTTTCCAATGATC | 1978-3768 | 660-1256 |
| A | 3 | GGTTGCTGGATCCATAAGAGATTTATACCCAGGTAAATTTTACTGGAGATTCTATGC | CCATGCTGAGCTCGCATTATTTATATTGATTAATCCTTTAACTAATTTACTATCTTCATCATAG | 3769-5556 | 1257-1852 |
| A | 4 | GGTTGCTGGATCCTCATTATTCTATTTTGATCCTATAGAATTTAACTTAGTAACTGGATGG | CCATGCTGAGCTCGCGCCATATATCCCAGGGGCTTTTACTCCATCAAC | 5557-8130 | 1853-2710 |
| B | 1 | GCTTGCTGGATCCAGTTTAGTTAATAGAAAACAGTTAGAAAAAATGG | CCATGCTGAGCTCGCTTCATAACTAATTTTATCTCCTTGTAACTG | 1-1638 | 1-546 |
| B | 2 | GCTTGCTGGATCCGCAGCATGTAACTTATTTGCAAAG | CCATGCTGAGCTCGCTATATCATCAGTTACAGTATGACCTGAAC | 1639-3553 | 547-1184 |
| B | 3 | GCTTGCTGGATCCGATCACTTCTTTTCAGCACCATC | CCATGCTGAGCTCGCCAAAGTCTTATCTTTAAAAACATTAACGAATC | 3553-5328 | 1185-1776 |
| B | 4 | GGTTGCTGGATCCGCAAATAAGCTATCTTTTAACTTTAGTGATAAACAAGATGTACC | CCATGCTGAGCTCGCTTCACTAATCACTAATTGAGCTGTATCAGGATCAAAATAATAC | 5329-7098 | 1777-2366 |
a
Underlining denotes the introduction of restriction sites to facilitate molecular cloning.