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Effect of tsetse fly saliva on the systemic immune response at 3 and 6 days after infection of TLR-2/4−/− mice. (A) Relative S12-normalized IL-6, IL-12, IFN-γ, iNOS, TNF, and IL-10 mRNA levels measured by RT-QPCR in RNA extracts from the white blood cell fraction. (B) Serum IFN-γ, TNF, and IL-10 cytokine concentrations measured by specific capture ELISAs. The presented data are representative of two independent infection experiments with four mice per group per time point.
