FIG. 4.
Intracellular survival of B. pseudomallei in BMM from BALB/c and C57BL/6 mice. (A) Unstimulated macrophages infected with an MOI of 1:1. (B) IFN-γ-stimulated (375 U/ml) macrophages infected with an MOI of 1:1. (C) IFN-γ-stimulated (375 U/ml) macrophages infected with an MOI of 50:1. (D) Survival of B. pseudomallei 24 h after infection (MOI, 50:1) of macrophages stimulated with different doses of IFN-γ. There was no significant difference between the values of cells stimulated with either 25 U or 375 U per ml at 0 h (data not shown). Values are the means ± standard deviations from at least duplicate determinations. Representative experiments are shown. p.i., postinfection.