FIG. 3.
The rise in UspA2 antibodies was directed against UspA2200-480 containing conserved areas that share identity with UspA1. Human sera were diluted in 1:100. The horizontal bars represent median values. All values are the averages of two separate ELISAs performed independently. (A) Most children in the younger group (6 months to 1 year old) had low IgG reactivity towards UspA2. (B) In the older group (2 to 3 years old), there were more children with higher reactivity towards some regions of UspA2, although median values remain low. (C) A clear pattern of reactivity against the conserved and shared regions of UspA2 was seen in the healthy adult population. (D) Recombinant truncated UspA2 fragments spanning the entire protein were used as antigens in ELISAs. Abbreviations: abs, antibodies; OD450, optical density at 450 nm.