FIG. 5.
Analysis of the IgG reactivity against MID, UspA1, and UspA2 in acute- and convalescent-phase sera from four patients. (A) Significant rises in IgG antibodies directed against the adhesive domain MID764-913 were found in the convalescent (conv) sera (arrow). (B) Significant rises in IgG antibodies directed against UspA1360-680 were found in the convalescent-phase sera. Smaller constituent fragments, such as those of the fibronectin-binding domain UspA1299-452 (arrow), were immunodominant. (C) Significant rises in IgG antibodies directed against UspA2200-480 were found in the convalescent-phase sera. Smaller constituent fragments spanning this region, including the fibronectin-binding domain UspA2162-318 (arrow), were also reactive. These ELISAs were performed as described for Fig. 3. Mean values of two separate ELISAs are shown. Error bars indicate standard deviations.