FIG. 4.

Histologic and ultrastructure micrographs of infected tissue. The 21-day wild-type C. rodentium (WT CR)-infected tissue (B and B") is paired with 7-day ΔescN C. rodentium (ΔescN CR)-infected mouse tissue (A and A"). Toluidine blue (A and B) and electron micrographs (A′ and B′) of ΔescN and wild-type C. rodentium-infected tissue are shown. The A" and B" images are higher magnifications of the apical junction complexes in the A′ and B′ micrographs. The asterisk indicates a location of cell infiltration in the inflamed epithelium. TJ, tight junction; AJ, adherens junction; DS, desmosome. Scale bars, 50 μm (A), 1.0 μm (A′), and 0.2 μm (A").