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. 2006 Sep 5;74(11):6075–6084. doi: 10.1128/IAI.00721-06

FIG. 6.

FIG. 6.

Barrier permeability micrographs. (A) Phase images of low magnification of 21-day ΔescN C. rodentium (ΔescN CR)-infected and wild-type C. rodentium (WT CR)-infected tissue pretreated with a biotin tracer to assess barrier permeability. Biotin is held to the luminal border in both ΔescN C. rodentium-infected and wild-type C. rodentium-infected tissue. (B) Higher magnification of biotin- and DAPI-treated murine tissue that was infected with ΔescN C. rodentium or wild-type C. rodentium for 21 days. Double-headed arrows demonstrate the difference in crypt depth between ΔescN C. rodentium-infected and wild-type C. rodentium-infected tissue to indicate that the disease phenotype has not resolved at this time point. The asterisks in the DAPI and merged images indicate some of the regions with high levels of cellular infiltrate in the 21-day wild-type C. rodentium-infected inflamed epithelium. Arrowheads point to regions with normal cellular load in the lamina propria. Scale bars, 100 μm (low magnification) and 50 μm (high magnification).