FIG. 7.

(A) Paired phase, C. rodentium Tir, and DAPI micrographs of infected tissue. Tir and DAPI colocalization demonstrates that bacteria labeled by DAPI in infected tissue sections is C. rodentium. Scale bar, 50 μm. (B) Paired phase, claudin-3, and DAPI micrographs of uninfected and 14-day wild-type C. rodentium (WT CR)-infected tissue. Arrows indicate regions of the epithelium without bacterial attachment that label the lateral boundaries of colonocytes. Arrowheads and white asterisks identify regions with bacterial colonization and direct attachment to colonocytes. All infected regions have altered localization of claudin-3. The black asterisks in the DAPI and merged images indicate areas with high levels of cellular infiltrate. Scale bar, 50 μm.