TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain/plasmid | Relevant phenotype, description, and/or selective markera | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | F′ φ80dlacZ ΔM15 Δ(lacZYA · argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Takara Bio, Otsu, Japan |
| Porphyromonas gingivalis | ||
| ATCC 33277 | Wild type | American Type Culture Collection |
| ATCC 33277 htpG strain | Ermr | This study |
| ATCC 33277 wecA strain | Ermr | This study |
| ATCC 33277 wbaP strain | Ermr | This study |
| ATCC 33277 galE strain | Ermr | This study |
| ATCC 33277 galE-c strain | Tetr Erms | This study |
| ATCC 33277 wzt strain | Ermr | This study |
| Plasmids | ||
| pUC19 | Cloning vector; Ampr | Takara Bio, Otsu, Japan |
| pBR322 | Cloning vector; Ampr | Takara Bio, Otsu, Japan |
| pHS19 | Contains the ermF-ermAM cassette between EcoRI and BamHI sites of pBR322 without bla; Ermr | 10 |
| pBR322-erm | Contains the ermF-ermAM cassette between EcoRI and BamHI sites of pBR322; Ampr Ermr | This study |
| pRN2 | Contains PG0045 between KpnI and AvaI sites of pUC19; Ampr | This study |
| pRN2-erm | Contains the ermF-ermAM cassette in PmaCI site within PG0045 of pRN2; Ampr Ermr | This study |
| pRN3 | Contains PG0106 between XbaI and BamHI sites of pUC19; Ampr | This study |
| pRN3-erm | Contains the ermF-ermAM cassette in BglII site within PG0106 of pRN3; Ampr Ermr | This study |
| pRN4 | Contains PG1964 between EcoRI and XbaI sites of pUC19; Ampr | This study |
| pRN4-erm | Contains the ermF-ermAM cassette in Bsp1407I site within PG1964 of pRN4; Ampr Ermr | This study |
| pRN6 | Contains PG0347 between EcoRI and BamHI sites of pUC19; Ampr | This study |
| pRN6-erm | Contains the ermF-ermAM cassette in BseRI site within PG0347 of pRN6; Ampr Ermr | This study |
| pRN7 | Contains PG1225 between XbaI and BamHI sites of pUC19; Ampr | This study |
| pRN7-erm | Contains the ermF-ermAM cassette in ApaI site within PG1225 of pRN7; Ampr Ermr | This study |
| pKD375 | Contains tetQ cassette in pUC19; Ampr Tetr | 25 |
| pUC19Q | Contains tetQ cassette between SmaI and BamHI sites in pUC19; Ampr Tetr | This study |
| pQG | Contains an entire region of galE and the 0.3-kb downstream region between BamHI and XbaI sites of pUC19Q; Ampr Tetr | This study |
| pGQG | Contains a region of 0.8 kb upstream of galE between KpnI and SmaI sites of pQG; Ampr Tetr | This study |
a
Ampr, ampicillin resistant; Ermr, erythromycin resistant; Erms, erythromycin sensitive; Tetr, tetracycline resistant.