Skip to main content
. 2006 Sep 5;74(11):6196–6205. doi: 10.1128/IAI.00753-06

FIG. 1.

FIG. 1.

The EHEC O157:H7 carboxy terminus is important for Tir phosphorylation. (A) Deletion mutations described in the text. (B) Anti-Tir immunoblot of bacterial pellets derived from EHECΔtir expressing WT Tir or the deletion mutants shown in panel A. (C) Anti-Tir immunoblot of TX-100-soluble fractions from HeLa cells incubated with EHECΔtir expressing WT Tir or the deletion mutants shown in panel A. CVD451 is a type III secretion mutant and does not translocate Tir. Arrowheads indicate phosphorylated Tir. In panels B and C, apparent molecular masses are in kilodaltons.