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EHEC O157:H7 Tir amino acids 452 to 463 are required for pedestal formation. (A) Immunofluorescence micrograph of HeLa cells infected with EHEC Tir mutants deficient for pedestal formation. Cells are triple labeled with anti-O157, anti-EHEC Tir, and phalloidin. (B) Anti-Tir immunoblot of TX-100-soluble fractions from HeLa cells infected with EHECΔtir expressing WT Tir or the deletion mutants. Apparent molecular mass markers are in kilodaltons.
