FIG. 4.

Amino acids 451 to 459 are required for Nck-independent pedestal formation by EPEC Tir. (A) Alignment of EHEC O157:H7 and EPEC C-terminal Tir sequences within the regions of the deletions described within the text. Phosphorylated tyrosines within EPEC Tir are indicated by asterisks, while the conserved region deleted within EPEC Tir is underlined. (B) Immunofluorescence micrograph of HeLa cells infected with either EHECΔtir/EPEC Tir Y474F or EHECΔtir/EPEC Tir Y474F Δ451-459 and labeled for EPEC Tir, actin, and anti-O157. (C) HeLa cells infected with EHECΔtirEPEC Tir or EHECΔtirEPEC TirΔ451-459 and labeled for EPEC Tir, actin, and Nck.