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. 2006 Sep 5;74(11):6196–6205. doi: 10.1128/IAI.00753-06

FIG. 5.

FIG. 5.

The requirements for α-actinin recruitment differ between EHEC and EPEC Tirs. (A) Immunofluorescence micrograph of HeLa cells infected with EHECΔtir expressing either WT EHEC Tir or mutant Tir proteins unable to support pedestal formation. Cells were quadruple labeled with anti-O157, anti-EHEC Tir, anti-α-actinin, and phalloidin. (B) Immunofluorescence micrograph of HeLa cells infected with either EHECΔtir/EPEC Tir Y474F or the non-pedestal-forming mutant EHECΔtir/EPEC Tir Y474FΔ451-459. Cells were quadruple labeled for anti-O157, anti-EPEC Tir, anti-α-actinin, and phalloidin.