TABLE 2.
Oligonucleotides used in this studya
| Oligonucleotide | Sequence |
|---|---|
| Tir 403+ | GG ACT AGT GCA CGT ACG GTA |
| Tir 394− | GG ACT AGT TTG TTC TAC CGG |
| Tir 427+ | GG ACT AGT GAA GAT ACC ATG |
| Tir 405− | GG ACT AGT AGT TGT AGT TGT |
| Tir 442+ | GG ACT AGT TCG ACT TTC TTT GAC ACT T |
| Tir 425− | GG ACT AGT ATC TAC ATT GCC CTG TGC A |
| Tir 463+ | TTT ACT AGTCTG CAT GAT TCG CAG GTG CCG |
| Tir 442− | TTT ACT AGT GCT AGC CAT CGA GCT ACG TCT |
| Tir 472+ | TTT ACT AGT AAT TCT AAT ACG TCT GTT CAG AA |
| Tir 464− | TTT ACT AGT TTT AAC ATC AGC ATA CGG ATT C |
| Tir 490+ | GG 0ACT AGT ACC ATT CAA CAT |
| Tir 473− | GG ACT AGT CGG CAC CTG CGA |
| Tir 511+ | GG ACT AGT AGT GCG GGG ATT |
| Tir 486− | GG ACT AGT TGT ATT CCC CAT |
| Tir 449− | TTT ACT AGT CAG GTG CCG ACT TCT AAT TC |
| Tir 454− | TTT ACT AGT CCC TAT GCT GGA AGT GTC AA |
| Tir 467+ | TTT ACT AGT CAG GTG CCG ACT TCT AAT TC |
| Tir 463+ | TTT ACT AGT CTG CAT GAT TCG CAG GTG C |
| Tir 458+ | AAA GGT ACC GCT GAT GTT AAA ACA TCC C |
| Tir 454− | AAA GGT ACC TAT GCT GGA AGT GTC AAA G |
| Tir 450+ | AAA ACT AGT ATA GGG ACC GTG CAG AAT CCG |
| Tir 443− | AAA ACT AGT GCT AGC CAT CGA GCT ACG TCT GC |
| Tir 462+ | GG ACT AGT CTG CAT GAT TCG CAG GTG |
| Tir 458− | GG ACT AGT CGG ATT CTG CAC GGT CCC TAT GCT GG |
| Tir 453+ | AAA ACT AGT GTG CAG AAT CCG TAT GCT GAT G |
| Tir 450− | GG ACT AGT GTC AAA GAA AGT CGA CGA GG |
| TirΔPKA+ | TTT TCT AGA CGA GCC GCG ATG GCT AGC AC |
| TirΔPKA− | TTT TCT AGA CTC CAT GGT ATC TTC TGA CCC |
| TirY458F+ | ATG CAG ACT AGT TTT AAC ATC AGC AAA CGG ATT CTG |
| TirY458F− | GTT AAA ACT AGT CTG CAT GAT TCG CAG GTG |
| EPEC 451+ | TTT GCG GCG TGA CCA GTG TGT CGA TGC AAC |
| EPEC 459− | TTT GCG CGC CAC TTC GCT AGA GGA ACT TGA CC |
a
The sequences of introduced restriction sites are underlined.