TABLE 4.

Summary of Tir internal deletion mutant sequences and phenotypes^a

Mutant	Sequence	Focused Tir	Pedestal formation
TirΔ442-463	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ449-472	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ449-467	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ454-472	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ449-463	MASTSSTFFDTSSIGTVQNPYADVKTSLDHSQVPTS	Yes	No
TirΔ454-467	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ464-467	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	Yes
TirΔ442-450	MASTSSTFFDTSSIGTVQNPYADVKTSLDHSQVPTS	Yes	Yes
TirΔ454-463	MASTSSTFFDTSSIGTVQNPYADVKTSLDHSQVPTS	Yes	No
TirΔ458-462	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	No
TirΔ454-458	MASTSSTFFDTSSIGTVQNPYADVKTSLDHSQVPTS	Yes	No
TirΔ450-453	MASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTS	Yes	Yes
EPECΔ451-459	QGSVASTHWSDSSSEVVNPYAEVGGARNSLSAHQ	Yes	Yes
EPEC Y474F Δ451-459	QGSVASTHWSDSSSEVVNPYAEVGGARNSLSAHQ	Yes	No

^aAll constructs were expressed from a plasmid harbored by EHECΔtir. Deleted residues are underlined, and the residues that distinguish functional and nonfunctional Tir mutants are shown in boldface type.