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Primary culture of adult glioblastoma cells. (A) Tumor cells looking like neurosphere were found in three glioblastoma primary cultured cell lines in 10% FBS/DMEM/F-12 medium. (B) Neurosphere derived from a single isolated CD133 positive cell cultured in NSC medium. (C) CD133 expression on neurospheres derived from a single isolated CD133 positive cell in NSC medium. Red staining indicates CD133 positive. (Magnification = ×100).
