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Protein net charge of folded PGB1-QDD according to model pKa values (solid curve), pKa values in low salt (solid curve with solid circles), and pKa values in high salt (solid curve with open circles). Protein net charge of wt PGB1 according to model pKa values (dashed curve) and pKa values from (5) (solid curve with triangles). (Inset) pI for the different proteins and models.
