FIG. 1.
Killing of V. cholerae O1 classical biotype by glucose-induced pH drop. (A and B) Aerobic flask culture of El Tor biotype strain N16961 and classical biotype strain O395. Bacteria were cultured in LB containing 0% (LB) or 1% (LBG) glucose, and growth was monitored by measuring OD600. (C) Loss of viability in the glucose-supplemented culture of O395. To enumerate viable cells, 10 μl of serial dilutions (∼10−3 to 10−7) was spotted on LB agar plates and incubated at 37°C overnight. The left and right sides of each plate represent bacteria grown in LB and LBG, respectively, for 15 h. (D) pH change of the culture supernatants in the culture of N16961 or O395. Bacteria were grown in LB plus 1% glucose.