TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Serogroup/biotype | Relevant genotype or description | Reference or source |
|---|---|---|---|
| Strains | |||
| V. cholerae | |||
| N16961 | O1/El Tor | SmrlacZ | 8 |
| O395 | O1/classical | SmrlacZ | 8 |
| CA401 | O1/classical | 6 | |
| 569B | O1/classical | Smr | 6 |
| MO10 | O139 | Smr | 51 |
| AM19226 | Non-O1, non-O139 | Smr | 9 |
| V52 | Non-O1, non-O139 | Smr | 39 |
| SSY01 | N16961, als::TnKGL3 | This study | |
| SSY02 | N16961, Δald | This study | |
| SSY03 | N16961, ΔVC1591 | This study | |
| SSY04 | O395, pgi::TnKGL3 | This study | |
| E. coli | |||
| DH5α | supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 13 | |
| SM10λpir | Kmrthi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu pir+ | 5 | |
| BW20767 | Km::Tn7 leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 uidA(ΔMlu1)::pir+thi | 34 | |
| P. aeruginosa | |||
| PAO1 | 14 | ||
| Plasmids | |||
| pWM91 | sacB suicide vector, Apr | 34 | |
| pTnKGL3 | Suicide vector bearing TnKGL3, Cmr Kmr | This study | |
| pVIK112 | Suicide vector for lacZ reporter fusion, Kmr | 20 |