FIG. 3.

Cytokine and chemokine mRNA induction during Chlamydia infection. PPEC monolayers in 25-cm² flasks were infected at an MOI of 15 of C. muridarum as described in Materials and Methods. Total RNA was extracted at the indicated times after infection and analyzed by a qualitative RT-PCR to assess the expression of TNF-α, IL-1 β, IL-6, ICAM-1 (a), MIP-1α, MIP-1β, IL-8, MCP-1, RANTES, IP-10 (b), and β-actin in mock-infected and C. muridarum-infected cultures. Figures are representative of at least three experiments performed.