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MyD88-deficient peritoneal cells fail to produce IgM in response to B. hermsii in vitro. Peritoneal cells were treated with LPS (200 ng/ml), B. hermsii sonicate (50 μg/ml), or live B. hermsii (2.4 × 107) for 5 days at 37°C. Supernatants were collected, and total IgM production was measured by ELISA. Data bars represent the average IgM levels from five MyD88+/− mice and four MyD88−/− mice.
